Genome-Wide Identification, Characterization, and Expression Analysis of Small RNA Biogenesis Purveyors Reveal Their Role in Regulation of Biotic Stress Responses in Three Legume Crops

Garg, V and Agarwal, G and Pazhamala, L T and Nayak, S N and Kudapa, H and Khan, A W and Doddamani, D and Sharma, M and Kavi Kishor, P B and Varshney, R K (2017) Genome-Wide Identification, Characterization, and Expression Analysis of Small RNA Biogenesis Purveyors Reveal Their Role in Regulation of Biotic Stress Responses in Three Legume Crops. Frontiers in Plant Science, 8 (488). pp. 1-16. ISSN 1664-462X

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Abstract

Biotic stress in legume crops is one of the major threats to crop yield and productivity. Being sessile organisms, plants have evolved a myriad of mechanisms to combat different stresses imposed on them. One such mechanism, deciphered in the last decade, is small RNA (sRNA) mediated defense in plants. Small RNAs (sRNAs) have emerged as one of the major players in gene expression regulation in plants during developmental stages and under stress conditions. They are known to act both at transcriptional and post-transcriptional levels. Dicer-like (DCL), Argonaute (AGO), and RNA dependent RNA polymerase (RDR) constitute the major components of sRNA biogenesis machinery and are known to play a significant role in combating biotic and abiotic stresses. This study is, therefore, focused on identification and characterization of sRNA biogenesis proteins in three important legume crops, namely chickpea, pigeonpea, and groundnut. Phylogenetic analysis of these proteins between legume species classified them into distinct clades and suggests the evolutionary conservation of these genes across the members of Papillionidoids subfamily. Variable expression of sRNA biogenesis genes in response to the biotic stresses among the three legumes indicate the possible existence of specialized regulatory mechanisms in different legumes. This is the first ever study to understand the role of sRNA biogenesis genes in response to pathogen attacks in the studied legumes.

Item Type: Article
Divisions: Research Program : Genetic Gains
CRP: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: AGO, DCL, RDR, gene expression, biotic stress, papillionidoids, genome-wide identification, biogenesis proteins, chickpea, pigeonpea, groundnut, legume crops
Subjects: Mandate crops > Chickpea
Mandate crops > Pigeonpea
Mandate crops > Groundnut
Others > Genetics and Genomics
Others > Legume Crops
Depositing User: Mr Ramesh K
Date Deposited: 16 May 2017 09:51
Last Modified: 01 Jun 2017 09:14
URI: http://oar.icrisat.org/id/eprint/10009
Official URL: http://dx.doi.org/10.3389/fpls.2017.00488
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: This work has been undertaken as a part the CGIAR Research Program on Genetic Gains. ICRISAT is a member of the CGIAR consortium. VG acknowledges the award of Research Fellowship from Department of Science and Technology, Government of India.
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