Molecular characterization and expression analysis of pearl millet plasma membrane proteolipid 3 (Pmp3) genes in response to abiotic stress conditions

Yeshvekar, R K and Nitnavare, R B and Chakradhar, T and Bhatnagar-Mathur, P and Reddy, M K and Reddy, P S (2017) Molecular characterization and expression analysis of pearl millet plasma membrane proteolipid 3 (Pmp3) genes in response to abiotic stress conditions. Plant Gene, 10. pp. 37-44. ISSN 2352-4073

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Abstract

Plasma membrane proteolipid 3 (Pmp3) is a gene family involved in abiotic stress response and cellular protection. Here we report cloning of two genes PgPmp3-1 and PgPmp3-2 from Pennisetum glaucum, and characterization with respect to their functions and responsiveness to various abiotic stresses. Both PgPmp3-1 and PgPmp3-2 genes are 171 bp long and encode for 56 amino acid long peptides. PgPmp3 sequences share 70–99% sequence identity with their homologs. Protein secondary structure prediction revealed membrane-spanning regions containing a membrane potential modulator domain in both PgPmp3 proteins. In silico network analyses revealed Pmp3 co-expression and association with proteins conferring abiotic stress tolerance in plants. Expression profiles of PgPmp3-1 and PgPmp3-2 revealed their up-regulation in P. glaucum under cold and salt stresses, but showed reduced expression in response to heat stress. These findings provide insight into the role of P. glaucum Pmp3 in abiotic stress amelioration.

Item Type: Article
Divisions: Research Program : Genetic Gains
CRP: CGIAR Research Program on Dryland Cereals
Uncontrolled Keywords: Pennisetum glaucum; Plasma membrane proteolipid 3 (Pmp3); Low temperature; Abiotic stress; Gene expression; Network analysis
Subjects: Others > Molecular biology
Mandate crops > Millets > Pearl Millet
Others > Genetics and Genomics
Depositing User: Mr Ramesh K
Date Deposited: 15 May 2017 08:38
Last Modified: 15 May 2017 08:40
URI: http://oar.icrisat.org/id/eprint/10004
Official URL: http://doi.org/10.1016/j.plgene.2017.05.002
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: This work was supported partially by the Department of Biotechnology, Ministry of Science and Technology (Government of India) to MKR. PSR acknowledges the Department of Science and Technology (DST), Govt. of India for financial support through INSPIRE Faculty Program (Award No. IFA-LSPA-06) and Young Scientist Scheme (SB/YS/LS-12/2013).
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